RAD50 Polyklonaler Antikörper

RAD50 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29390-1-PBS

Synonyme

hRad50, RAD50 homolog (S. cerevisiae)

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  WB analysis using 29390-1-AP (same clone as 29390-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RAD50 antibody (29390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29390-1-AP (same clone as 29390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29390-1-AP (same clone as 29390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29390-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA RAD50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD50 fusion protein Ag29949
• Vollständiger Name: RAD50 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: 154 kDa
• GenBank-Zugangsnummer: NM_005732
• Gene symbol: RAD50
• Gene ID (NCBI): 10111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).