RAD50 Rekombinanter Antikörper

RAD50 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242541A5

Kat-Nr. : 84969-4-PBS

Synonyme

242541A5, hRad50, RAD50 homolog (S. cerevisiae)

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  WB analysis using 84969-4-RR (same clone as 84969-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84969-4-RR (RAD50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84969-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84969-4-RR against Human RAD50 were performed. The affinity constant is 6.79 nM.

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Geprüfte Anwendungen

Produktinformation

84969-4-PBS bindet in WB, IHC, Indirect ELISA RAD50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAD50 fusion protein Ag29949
• Vollständiger Name: RAD50 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: 154 kDa
• GenBank-Zugangsnummer: NM_005732
• Gene symbol: RAD50
• Gene ID (NCBI): 10111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).