RAD51 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAD51 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

63

Rezensionen (2)

Kat-Nr. : 14961-1-AP

Synonyme

BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA

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Galerie der Validierungsdaten

• 

  WB analysis using 14961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14961-1-AP

  WB result of RAD51 antibody (14961-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells.

• 

  WB analysis of mouse testis using 14961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, Maushodengewebe, MCF-7-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 53 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

14961-1-AP bindet in WB, CoIP, ChIP, ELISA RAD51 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD51 fusion protein Ag6823
• Vollständiger Name: RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC001459
• Gene symbol: RAD51
• Gene ID (NCBI): 5888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Publikationen

Species	Application	Title
mouse		Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	Nucleic Acids Res; LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA.; Authors - Liping Shen ; KD Validated
human	WB,CoIP	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou
human	WB	Int J Biol Macromol; Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation; Authors - Hong-Yu Tao
human	WB	Oncogene; BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair.; Authors - Mengdong Ni

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Resha (Verified Customer) (10-11-2023)

The antibody detected band at 37KDa for Rad51 protein. We used 1:1000 dilution for 20ug of protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

FH

ksh (Verified Customer) (01-17-2022)

This Antibody works for WB (1:1000) and IF (1:200).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

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