RAD51AP1 Polyklonaler Antikörper

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• KD/KO Validated

RAD51AP1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11255-1-PBS

Synonyme

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  WB analysis using 11255-1-AP (same clone as 11255-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11255-1-AP (same clone as 11255-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 11255-1-AP (same clone as 11255-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11255-1-AP (same clone as 11255-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11255-1-AP (same clone as 11255-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11255-1-AP (same clone as 11255-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11255-1-AP (RAD51AP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11255-1-PBS bindet in WB, IF/ICC, Indirect ELISA RAD51AP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD51AP1 fusion protein Ag1781
• Vollständiger Name: RAD51 associated protein 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC016330
• Gene symbol: RAD51AP1
• Gene ID (NCBI): 10635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].