RAD51AP1 Monoklonaler Antikörper

RAD51AP1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D8B1

Kat-Nr. : 68560-1-Ig

Synonyme

RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51

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  WB analysis using 68560-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68560-1-Ig (RAD51AP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, Daudi-Zellen, HaCaT-Zellen, Jurkat-Zellen, K-562-Zellen, NCCIT-Zellen, NIH/3T3-Zellen, SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68560-1-Ig bindet in WB, ELISA RAD51AP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAD51AP1 fusion protein Ag28385
• Vollständiger Name: RAD51 associated protein 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC016330
• Gene symbol: RAD51AP1
• Gene ID (NCBI): 10635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].