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RAD51C Rekombinanter Antikörper

RAD51C Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241971A11

Kat-Nr. : 84537-5-PBS

Synonyme

241971A11

Filter:
  • Western Blot (WB) analysis of various lysates using RAD51C Recombinant antibody (84537-5-RR)

    WB analysis using 84537-5-RR (same clone as 84537-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84537-5-RR (RAD51C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84537-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using RAD51C Recombinant antibody (84537-5-RR)

    FC experiment of MCF-7 using 84537-5-RR (same clone as 84537-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RAD51C Recombinant antibody (84537-5-RR, Clone:241971A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84537-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84537-5-RR

    Affinity and Kinetic Characterization of 84537-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84537-5-RR against Human RAD51C were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84537-5-PBS bindet in WB, FC (Intra), Indirect ELISA RAD51C und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAD51C fusion protein Ag35070
Vollständiger Name RAD51 homolog C (S. cerevisiae)
Berechnetes Molekulargewicht 376 aa, 42 kDa
Beobachtetes Molekulargewicht 40-42 kDa
GenBank-ZugangsnummerBC107753
Gene symbol RAD51C
Gene ID (NCBI) 5889
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).