RAD52 Polyklonaler Antikörper

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• KD/KO Validated

RAD52 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 28045-1-AP

Synonyme

DNA repair protein RAD52 homolog

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• 

  WB analysis using 28045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28045-1-AP (RAD52 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28045-1-AP

  WB result of RAD52 antibody (28045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with negative control and RAD52 knockout HeLa cells.

• 

  WB analysis of HEK-293 using 28045-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 28045-1-AP (RAD52 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 28045-1-AP

  IP result of anti-RAD52 (IP:28045-1-AP, 4ug; Detection:28045-1-AP 1:1000) with HEK-293 cells lysate 2960 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below

Produktinformation

28045-1-AP bindet in WB, IP, ELISA RAD52 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD52 fusion protein Ag27619
• Vollständiger Name: RAD52 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 46 aa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: NM_001297419
• Gene symbol: RAD52
• Gene ID (NCBI): 5893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Mol Cell; EXO1 protects BRCA1-deficient cells against toxic DNA lesions; Authors - Bert van de Kooij
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB	J Cell Mol Med; RDM1 promotes critical processes in breast cancer tumorigenesis.; Authors - Yajun Chen
pig	WB	Int J Biochem Cell Biol; Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.; Authors - Guoling Li
human	WB	Cell Death Dis; SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.; Authors - Yuchao Diao ; KD Validated
human	WB	Nat Commun; Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity; Authors - Ronan Broderick ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-15-2024)

I detected the expected band for RAD52 and 48 kDa in WT cells, but not RAD52 KO cells, proving that the antibody is indeed recognizing RAD52.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116 and RPE1 cells