RAD9A Polyklonaler Antikörper

RAD9A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13035-1-PBS

Synonyme

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• 

  WB analysis of HeLa using 13035-1-AP (same clone as 13035-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13035-1-AP (same clone as 13035-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 13035-1-AP (same clone as 13035-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13035-1-AP (same clone as 13035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13035-1-AP (same clone as 13035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13035-1-AP (same clone as 13035-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13035-1-AP (RAD9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13035-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RAD9A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD9A fusion protein Ag3687
• Vollständiger Name: RAD9 homolog A (S. pombe)
• Berechnetes Molekulargewicht: 391 aa, 42 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC014848
• Gene symbol: RAD9A
• Gene ID (NCBI): 5883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.