RADIL Polyklonaler Antikörper

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• KD/KO Validated

RADIL Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20284-1-AP

Synonyme

KIAA1849, Ras-associating and dilute domain-containing protein

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  WB analysis using 20284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20284-1-AP

  IP result of anti-RADIL (IP:20284-1-AP, 4ug; Detection:20284-1-AP 1:1000) with HEK-293 cells lysate 1400 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushodengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

20284-1-AP bindet in WB, IP, ELISA RADIL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RADIL fusion protein Ag13996
• Vollständiger Name: Ras association and DIL domains
• Berechnetes Molekulargewicht: 1075 aa, 117 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC004919
• Gene symbol: RADIL
• Gene ID (NCBI): 55698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Radil is a broadly expressed adapter protein that contains a RA-like domain, a PDZ domain and a DIL domain. Like RAPL and RIAM, Radil interacts preferentially with active GTP-bound Rap1, and overexpression of Radil enhances integrin-mediated adhesion. In addition, Radil knockdown inhibits cell-substrate adhesion in HT1080, AD293, and NMuMG cells and results in migration defects in SW1353 and zebrafish neural crest cells (PMID: 17704304). Radil regulates neutrophil adhesion by specifically enhancing β2-integrin activation, as well as focal adhesion kinase (FAK) and paxillin phosphorylation (PMID: 23097489).

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Proteomic Mapping of the Interactome of KRAS Mutants Identifies New Features of RAS Signalling Networks and the Mechanism of Action of Sotorasib; Authors - Aoife Nolan
human	WB	J Biol Chem; Identification of Radil as a Ras binding partner and putative activator.; Authors - Byeong Hyeok Choi ; KD Validated