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RADIL Polyklonaler Antikörper

RADIL Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20284-1-PBS

Synonyme

KIAA1849, Ras-associating and dilute domain-containing protein

Filter:
  • Western Blot (WB) analysis of various lysates using RADIL Polyclonal antibody (20284-1-AP)

    WB analysis using 20284-1-AP (same clone as 20284-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20284-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RADIL Polyclonal antibody (20284-1-AP)

    IP experiment of HEK-293 using 20284-1-AP (same clone as 20284-1-PBS)

    IP result of anti-RADIL (IP:20284-1-AP, 4ug; Detection:20284-1-AP 1:1000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 20284-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20284-1-PBS bindet in WB, IP, Indirect ELISA RADIL und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RADIL fusion protein Ag13996
Vollständiger Name Ras association and DIL domains
Berechnetes Molekulargewicht 1075 aa, 117 kDa
Beobachtetes Molekulargewicht 117 kDa
GenBank-ZugangsnummerBC004919
Gene symbol RADIL
Gene ID (NCBI) 55698
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Radil is a broadly expressed adapter protein that contains a RA-like domain, a PDZ domain and a DIL domain. Like RAPL and RIAM, Radil interacts preferentially with active GTP-bound Rap1, and overexpression of Radil enhances integrin-mediated adhesion. In addition, Radil knockdown inhibits cell-substrate adhesion in HT1080, AD293, and NMuMG cells and results in migration defects in SW1353 and zebrafish neural crest cells (PMID: 17704304). Radil regulates neutrophil adhesion by specifically enhancing β2-integrin activation, as well as focal adhesion kinase (FAK) and paxillin phosphorylation (PMID: 23097489).