RAE1 Rekombinanter Antikörper

RAE1 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240861E12

Kat-Nr. : 83712-1-PBS

Synonyme

240861E12, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1

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  WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83712-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83712-1-PBS against Human RAE1 was performed. The affinity constant is 1.14 nM.

• 

  Affinity and Kinetic Characterization of 83712-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

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Geprüfte Anwendungen

Produktinformation

83712-1-PBS bindet in WB, FC (Intra), Indirect ELISA RAE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAE1 fusion protein Ag12102
• Vollständiger Name: RAE1 RNA export 1 homolog (S. pombe)
• Berechnetes Molekulargewicht: 368 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC103754
• Gene symbol: RAE1
• Gene ID (NCBI): 8480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)