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RAE1 Rekombinanter Antikörper

RAE1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240861B7

Kat-Nr. : 83712-4-PBS

Synonyme

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

Filter:
  • Western Blot (WB) analysis of various lysates using RAE1 Recombinant antibody (83712-4-RR)

    WB analysis using 83712-4-RR (same clone as 83712-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-4-RR (RAE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAE1 Recombinant antibody (83712-4-RR)

    IHC staining of mouse testis using 83712-4-RR (same clone as 83712-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83712-4-RR (RAE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAE1 Recombinant antibody (83712-4-RR)

    IF Staining of HepG2 using 83712-4-RR (same clone as 83712-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAE1 antibody (83712-4-RR, Clone: 240861B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83712-4-RR

    Affinity and Kinetic Characterization of 83712-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-4-RR against Human RAE1 were performed. The affinity constant is 20.7 nM.

Geprüfte Anwendungen

Produktinformation

83712-4-PBS bindet in WB, IHC, IF/ICC, ELISA RAE1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAE1 fusion protein Ag12102
Vollständiger Name RAE1 RNA export 1 homolog (S. pombe)
Berechnetes Molekulargewicht 368 aa, 41 kDa
GenBank-ZugangsnummerBC103754
Gene symbol RAE1
Gene ID (NCBI) 8480
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)