RAF1 Polyklonaler Antikörper

RAF1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26863-1-PBS

Synonyme

Proto-oncogene c-RAF, Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF

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  WB analysis of DU 145 using 26863-1-AP (same clone as 26863-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 26863-1-AP (RAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26863-1-AP (same clone as 26863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26863-1-AP (same clone as 26863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26863-1-AP (same clone as 26863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26863-1-PBS bindet in WB, IHC, ELISA RAF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAF1 fusion protein Ag25423
• Vollständiger Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Berechnetes Molekulargewicht: 648 aa, 73 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC018119
• Gene symbol: RAF1
• Gene ID (NCBI): 5894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.