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RAF1 Rekombinanter Antikörper

RAF1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242540A2

Kat-Nr. : 84994-4-PBS

Synonyme

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

Filter:
  • Western Blot (WB) analysis of various lysates using RAF1 Recombinant antibody (84994-4-RR)

    WB analysis using 84994-4-RR (same clone as 84994-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84994-4-RR (RAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAF1 Recombinant antibody (84994-4-RR)

    IF Staining of HeLa using 84994-4-RR (same clone as 84994-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAF1 antibody (84994-4-RR, Clone: 242540A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84994-4-RR

    Affinity and Kinetic Characterization of 84994-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84994-4-RR against Human RAF1 were performed. The affinity constant is 2.84 nM.

Geprüfte Anwendungen

Produktinformation

84994-4-PBS bindet in WB, IF/ICC, Indirect ELISA RAF1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAF1 fusion protein Ag25423
Vollständiger Name v-raf-1 murine leukemia viral oncogene homolog 1
Berechnetes Molekulargewicht 648 aa, 73 kDa
Beobachtetes Molekulargewicht~75 kDa
GenBank-ZugangsnummerBC018119
Gene symbol RAF1
Gene ID (NCBI) 5894
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.