RAF1 Rekombinanter Antikörper

RAF1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242540A2

Kat-Nr. : 84994-4-RR

Synonyme

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

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  WB analysis using 84994-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84994-4-RR (RAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84994-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAF1 antibody (84994-4-RR, Clone: 242540A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84994-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84994-4-RR against Human RAF1 were performed. The affinity constant is 2.84 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, DU 145-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84994-4-RR bindet in WB, IF/ICC, ELISA RAF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAF1 fusion protein Ag25423
• Vollständiger Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Berechnetes Molekulargewicht: 648 aa, 73 kDa
• Beobachtetes Molekulargewicht: ~75 kDa
• GenBank-Zugangsnummer: BC018119
• Gene symbol: RAF1
• Gene ID (NCBI): 5894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.