RAG2 Polyklonaler Antikörper

RAG2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11825-1-AP

Synonyme

RAG 2, RAG2

---

Galerie der Validierungsdaten

• 

  WB analysis of A375 using 11825-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11825-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11825-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11825-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11825-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 11825-1-AP

  IP Result of anti-RAG2 (IP:11825-1-AP, 4ug; Detection:11825-1-AP 1:300) with A375 cells lysate 3600ug.

• 

  IHC staining of human lymphoma using 11825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11825-1-AP (RAG2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11825-1-AP

  Immunofluorescent analysis of Hela cells, using RAG2 antibody 11825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 11825-1-AP

  1X10^6 HeLa cells were stained with 0.2ug RAG2 antibody (11825-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11825-1-AP bindet in WB, IP, IHC, IF, FC, ELISA RAG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAG2 fusion protein Ag2393
• Vollständiger Name: recombination activating gene 2
• Berechnetes Molekulargewicht: 527 aa, 59 kDa
• Beobachtetes Molekulargewicht: 57-62 kDa
• GenBank-Zugangsnummer: BC022397
• Gene symbol: RAG2
• Gene ID (NCBI): 5897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; The 3D enhancer network of the developing T cell genome is shaped by SATB1; Authors - Tomas Zelenka
mouse	WB	J Immunol; Allelic Exclusion of IgH through Inhibition of E2A in a VDJ Recombination Complex.; Authors - Jannek Hauser