RAG2 Polyklonaler Antikörper

RAG2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11825-1-PBS

Synonyme

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  WB analysis of A375 using 11825-1-AP (same clone as 11825-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11825-1-AP (same clone as 11825-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 11825-1-AP (same clone as 11825-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A375 using 11825-1-AP (same clone as 11825-1-PBS)

  IP result of anti-RAG2 (IP:11825-1-AP, 4ug; Detection:11825-1-AP 1:300) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11825-1-AP (same clone as 11825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11825-1-AP (RAG2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11825-1-AP (same clone as 11825-1-PBS)

  Immunofluorescent analysis of Hela cells, using RAG2 antibody 11825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11825-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11825-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RAG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAG2 fusion protein Ag2393
• Vollständiger Name: recombination activating gene 2
• Berechnetes Molekulargewicht: 527 aa, 59 kDa
• Beobachtetes Molekulargewicht: 57-62 kDa
• GenBank-Zugangsnummer: BC022397
• Gene symbol: RAG2
• Gene ID (NCBI): 5897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3