RAI14 Polyklonaler Antikörper

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• KD/KO Validated

RAI14 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 17507-1-AP

Synonyme

retinoic acid induced 14, RAI13, NORPEG, KIAA1334, Ankyrin repeat and coiled-coil structure-containing protein

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  WB analysis of HeLa using 17507-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 17507-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17507-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17507-1-AP (RAI14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 17507-1-AP

  IP result of anti-RAI14 (IP:17507-1-AP, 5ug; Detection:17507-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human stomach cancer using 17507-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17507-1-AP (RAI14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17507-1-AP

  Immunofluorescent analysis of Hela cells, using RAI14 antibody 17507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Plazenta-Gewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

17507-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RAI14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAI14 fusion protein Ag11708
• Vollständiger Name: retinoic acid induced 14
• Berechnetes Molekulargewicht: 980 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC052988
• Gene symbol: RAI14
• Gene ID (NCBI): 26064
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
	IF	EMBO Rep; Retinoic acid-induced protein 14 links mechanical forces to Hippo signaling; Authors - Wonyoung Jeong
human,mouse	WB,IP	Elife; Retinoic acid-induced protein 14 controls dendritic spine dynamics associated with depressive-like behaviors.; Authors - Soo Jeong Kim
mouse	WB,IF	Cells; Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.; Authors - Natalie Längst
Human	WB	Front Pharmacol; High Expression of RAI14 in Triple-Negative Breast Cancer Participates in Immune Recruitment and Implies Poor Prognosis Through Bioinformatics Analyses.; Authors - Ranliang Cui
human	WB	Cell Transplant; RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer.; Authors - XinXin Yan