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RAI14 Rekombinanter Antikörper

RAI14 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251495G12

Kat-Nr. : 86581-1-PBS

Synonyme

Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, KIAA1334, NORPEG, RAI13

Filter:
  • Western Blot (WB) analysis of various lysates using RAI14 Recombinant monoclonal antibody (86581-1-RR)

    WB analysis using 86581-1-RR (same clone as 86581-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using RAI14 Recombinant monoclonal antibody (86581-1-RR)

    WB analysis of rat testis using 86581-1-RR (same clone as 86581-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAI14 Recombinant monoclonal antibody (86581-1-RR)

    IF Staining of HeLa using 86581-1-RR (same clone as 86581-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAI14 antibody (86581-1-RR, Clone: 251495G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86581-1-PBS bindet in WB, IF/ICC, Indirect ELISA RAI14 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAI14 fusion protein Ag11708
Vollständiger Name retinoic acid induced 14
Berechnetes Molekulargewicht 980 aa, 110 kDa
Beobachtetes Molekulargewicht 110 kDa
GenBank-ZugangsnummerBC052988
Gene symbol RAI14
Gene ID (NCBI) 26064
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAI14 (retinoic acid-induced protein 14, also called ankycorbin or NORPEG) was originally identified in human retinal pigment epithelial cells as a retinoic-acid-responsive gene. Through direct interaction with actin-binding partners such as palladin and TARA, RAI14 orchestrates focal adhesion dynamics, cell polarity and mechano-sensing; in epithelia it stabilizes tight-junction complexes, whereas in neurons it localizes to the dendritic-spine neck where it promotes spine maintenance by antagonizing F-actin depolymerization. Consistent with its cytoskeletal role, RAI14 has emerged as a context-dependent modulator of several signalling cascades.