RAI14 Rekombinanter Antikörper

RAI14 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251495G12

Kat-Nr. : 86581-1-RR

Synonyme

Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, KIAA1334, NORPEG, RAI13

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  WB analysis using 86581-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 86581-1-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86581-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAI14 antibody (86581-1-RR, Clone: 251495G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86581-1-RR bindet in WB, IF/ICC, ELISA RAI14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAI14 fusion protein Ag11708
• Vollständiger Name: retinoic acid induced 14
• Berechnetes Molekulargewicht: 980 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC052988
• Gene symbol: RAI14
• Gene ID (NCBI): 26064
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAI14 (retinoic acid-induced protein 14, also called ankycorbin or NORPEG) was originally identified in human retinal pigment epithelial cells as a retinoic-acid-responsive gene. Through direct interaction with actin-binding partners such as palladin and TARA, RAI14 orchestrates focal adhesion dynamics, cell polarity and mechano-sensing; in epithelia it stabilizes tight-junction complexes, whereas in neurons it localizes to the dendritic-spine neck where it promotes spine maintenance by antagonizing F-actin depolymerization. Consistent with its cytoskeletal role, RAI14 has emerged as a context-dependent modulator of several signalling cascades.