RALA Polyklonaler Antikörper

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• KD/KO Validated

RALA Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 13629-1-AP

Synonyme

RAL, RALA, Ras related protein Ral A

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13629-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13629-1-AP

  WB result of RALA antibody (13629-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells.

• 

  WB analysis of rat brain using 13629-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13629-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 13629-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of MCF-7 using 13629-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human breast cancer using 13629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13629-1-AP (RALA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13629-1-AP (RALA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, HeLa-Zellen, humanes Hirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

13629-1-AP bindet in WB, IHC, ELISA RALA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RALA fusion protein Ag4547
• Vollständiger Name: v-ral simian leukemia viral oncogene homolog A (ras related)
• Berechnetes Molekulargewicht: 206 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC039858
• Gene symbol: RALA
• Gene ID (NCBI): 5898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RALA belongs to the small GTPase superfamily. Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. RALA plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. As an effector of the proto-oncogene Ras, it play an important role in the molecular pathology of human malignancies.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
human	WB, IHC	Front Chem; Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma.; Authors - Yuting Wang
human	IHC	Hum Pathol; Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors; Authors - Bohyun Kim
human	WB	Intractable Rare Dis Res; A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells.; Authors - Liang Jiang
mouse	WB	Oncotarget; Evaluation of cancer-derived myocardial impairments using a mouse model.; Authors - Yoshihiro Miyagawa