RALA Monoklonaler Antikörper

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• KD/KO Validated

RALA Monoklonal Antikörper für Indirect ELISA, IHC, WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

Indirect ELISA, IHC, WB

Konjugation

Unkonjugiert

CloneNo.

1H9H5

Kat-Nr. : 67093-1-PBS

Synonyme

Ras-related protein Ral-A, Ras related protein Ral A, RAL, EC:3.6.5.2, 1H9H5

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  WB analysis using 67093-1-Ig (same clone as 67093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67093-1-Ig (same clone as 67093-1-PBS)

  WB result of RALA antibody (67093-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67093-1-Ig (same clone as 67093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67093-1-Ig (same clone as 67093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67093-1-Ig (same clone as 67093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 67093-1-Ig (same clone as 67093-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RALA (67093-1-Ig, Clone:1H9H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67093-1-PBS bindet in Indirect ELISA, IHC, WB RALA und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RALA fusion protein Ag5329
• Vollständiger Name: v-ral simian leukemia viral oncogene homolog A (ras related)
• Berechnetes Molekulargewicht: 206 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC039858
• Gene symbol: RALA
• Gene ID (NCBI): 5898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.