RAMP1 Polyklonaler Antikörper

RAMP1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10327-1-AP

Synonyme

RAMP1

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Galerie der Validierungsdaten

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  WB analysis using 10327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10327-1-AP (RAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10327-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10327-1-AP (RAMP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10327-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10327-1-AP (RAMP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 10327-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10327-1-AP (RAMP1 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HepG2 using 10327-1-AP

  1X10^6 HepG2 cells were stained with .2ug RAMP1 antibody (10327-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

10327-1-AP bindet in WB, IHC, IF, FC, ELISA RAMP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAMP1 fusion protein Ag0402
• Vollständiger Name: receptor (G protein-coupled) activity modifying protein 1
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 14 kDa, 35 kDa
• GenBank-Zugangsnummer: BC000548
• Gene symbol: RAMP1
• Gene ID (NCBI): 10267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAMP1 (receptor-activity-modifying protein) is a member of the RAMP family of single-transmembrane-domain proteins which consist of an N-terminal extracellular domain, a transmembrane region, and a short intracellular C-terminal tail. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a G protein-coupled receptor, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. RAMP1 transports the CRLR to the plasma membrane and then remains associated with it to function as a terminally glycosylated CGRP receptor, while RAMP2 and RAMP3 transfer the CRLR to the cell surface to generate receptors that are preferentially selective for adrenomedullin. RAMP1 can form a homodimer which migrates at 30-37 kDa on SDS-PAGE. (PMID: 9620797; 16188935; 12051717;PMID: 18384073)

Publikationen

Species	Application	Title
rat	IF	J Headache Pain; Calcitonin gene-related peptide receptor antagonist BIBN4096BS regulates synaptic transmission in the vestibular nucleus and improves vestibular function via PKC/ERK/CREB pathway in an experimental chronic migraine rat model.; Authors - Ruimin Tian
rat	WB,IHC	Front Pharmacol; Effect of Xiongmatang Extract on Behavioral and TRPV1-CGRP/CGRP-R Pathway in Rats With Migraine.; Authors - Dingding Liu
rat	IF	Neurosci Lett; Calcitonin gene-related peptide differentially regulates gene and protein expression in trigeminal glia cells: findings from array analysis.; Authors - Vause Carrie V CV