RANBP10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RANBP10 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21107-1-AP

Synonyme

KIAA1464, RAN binding protein 10, RANBP10

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 21107-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21107-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21107-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 21107-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21107-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21107-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, LNCaP-Zellen, Mausherzgewebe, Maushirngewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

21107-1-AP bindet in WB, IHC, IF, ELISA RANBP10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RANBP10 fusion protein Ag15387
• Vollständiger Name: RAN binding protein 10
• Berechnetes Molekulargewicht: 620 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67-70 kDa
• GenBank-Zugangsnummer: BC099917
• Gene symbol: RANBP10
• Gene ID (NCBI): 57610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Theranostics; miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.; Authors - Lu-Shiun Her
human	WB	PLoS Pathog; A Ran-binding protein facilitates nuclear import of human papillomavirus type 16.; Authors - Kun-Yi Lai ; KD Validated