RANBP3 Polyklonaler Antikörper

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• KD/KO Validated

RANBP3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27168-1-AP

Synonyme

Ran-binding protein 3, RAN binding protein 3

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  WB analysis using 27168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27168-1-AP (RANBP3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27168-1-AP

  WB result of RANBP3 antibody (27168-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANBP3 transfected Jurkat cells.

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  IHC staining of mouse testis using 27168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27168-1-AP (RANBP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27168-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27168-1-AP (RANBP3 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 27168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RANBP3 antibody (27168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27168-1-AP bindet in WB, IHC, IF/ICC, ELISA RANBP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RANBP3 fusion protein Ag26000
• Vollständiger Name: RAN binding protein 3
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC004349
• Gene symbol: RANBP3
• Gene ID (NCBI): 8498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RANBP3 (Ran-binding protein 3) is also named as Rab escort protein 1 (REP-1). RanBP3 is a RanGTP‐binding protein that has been reported to enhance nuclear export of active β‐catenin, independent of its role as a CRM1‐associated nuclear export cofactorl (PMID: 9637251). RanBP3 has been reported to be a crucial downstream molecule of MAPK-ERK and PI3K-AKT signal pathways (PMID: 18331709). RanBP3 is a tumor suppressor in CRC and is associated with patient survival (PMID: 36270974). RanBP3 has been reported as a potential regulator in melanoma biology, hepatocellular carcinoma, prostate cancer, and ovarian cancer, and RanBP3 is highly expressed in CML Cells (PMID: 34504783).