RANBP9 Polyklonaler Antikörper

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• KD/KO Validated

RANBP9 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17755-1-AP

Synonyme

RanBP7, Ran-binding protein M, Ran-binding protein 9, BPM-L, BPM90

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  WB analysis of HeLa using 17755-1-AP

  WB result of RANBP9 antibody (17755-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANBP9 transfected HeLa cells.

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  WB analysis using 17755-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17755-1-AP (RANBP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17755-1-AP

  IP result of anti-RANBP9 (IP:17755-1-AP, 4ug; Detection:17755-1-AP 1:600) with HeLa cells lysate 1600ug.

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  IHC staining of human testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 17755-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RANBP9 antibody (17755-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of HeLa using 17755-1-AP

  Immunofluorescent analysis of HeLa cells using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hautgewebe, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17755-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RANBP9 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RANBP9 fusion protein Ag12112
• Vollständiger Name: RAN binding protein 9
• Berechnetes Molekulargewicht: 388aa,43 kDa; 729aa,78 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC063849
• Gene symbol: RANBP9
• Gene ID (NCBI): 10048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	mSphere; B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition.; Authors - Yan Chen
human	WB	Oncol Lett; RANBP9 suppresses tumor proliferation in colorectal cancer.; Authors - Chunzhi Qin
rat	IF	Acta Histochem; MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9.; Authors - Huiyuan Zhang
human	IHC	Transl Cancer Res; Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients.; Authors - Guangbin Wu
mouse	WB	Nat Commun; Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth; Authors - Nelson V Simwela