TNFSF11/RANKL Polyklonaler Antikörper

TNFSF11/RANKL Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(73)

Kat-Nr. : 23408-1-AP

Synonyme

TNFSF11, Tumor necrosis factor ligand superfamily member 11, TNF-related activation-induced cytokine, Receptor activator of nuclear factor kappa-B ligand, CD254

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  WB analysis using 23408-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23408-1-AP (RANKL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolongewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 48 publications below
• IHC: See 25 publications below
• IF: See 10 publications below

Produktinformation

23408-1-AP bindet in WB, IHC, IF, ELISA TNFSF11/RANKL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFSF11/RANKL fusion protein Ag19975
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 11
• Berechnetes Molekulargewicht: 317 aa, 35 kDa
• Beobachtetes Molekulargewicht: 20-30 kDa
• GenBank-Zugangsnummer: BC074890
• Gene symbol: RANKL
• Gene ID (NCBI): 8600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p.; Authors - Lijuan Yu
mouse,human	WB	Aging Cell; Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation; Authors - Jie Li
rat	IF	J Control Release; Sialic acid-modified chitosan oligosaccharide-based biphasic calcium phosphate promote synergetic bone formation in rheumatoid arthritis therapy.; Authors - Xiao-Ling Xu
mouse	WB	Antioxid Redox Signal; Titanium Particles Activate Osteocytic Connexin 43 to Induce Oxidative Stress and Osteoclastogenesis Through the JAK-STAT Pathway; Authors - Jiawei Ouyang
mouse	WB	Mol Metab; Loss of chemerin triggers bone remodeling in vivo and in vitro.; Authors - Long Han
mouse	WB	Aging (Albany NY); Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model.; Authors - Chuangxin Lin