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RAP1A Rekombinanter Antikörper

RAP1A Rekombinant Antikörper für ELISA, FC (Intra), WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

ELISA, FC (Intra), WB

Konjugation

Unkonjugiert

CloneNo.

240438E10

Kat-Nr. : 83708-2-PBS

Synonyme

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

Filter:
  • Western Blot (WB) analysis of various lysates using RAP1A Recombinant antibody (83708-2-RR)

    WB analysis using 83708-2-RR (same clone as 83708-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83708-2-RR (RAP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RAP1A Recombinant antibody (83708-2-RR)

    FC experiment of HeLa using 83708-2-RR (same clone as 83708-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-2-RR, Clone:240438E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83708-2-RR

    Affinity and Kinetic Characterization of 83708-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-2-RR against Human RAP1A were performed. The affinity constant is 0.232 nM.

Geprüfte Anwendungen

Produktinformation

83708-2-PBS bindet in ELISA, FC (Intra), WB RAP1A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAP1A fusion protein Ag9488
Vollständiger Name RAP1A, member of RAS oncogene family
Berechnetes Molekulargewicht 184 aa, 21 kDa
GenBank-ZugangsnummerBC014086
Gene symbol RAP1A
Gene ID (NCBI) 5906
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.