RAP1GAP Rekombinanter Antikörper

RAP1GAP Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250422B8

Kat-Nr. : 86011-1-RR

Synonyme

KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase-activating protein 1, RAP1GA1, RAP1GAP1

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  IF Staining of A549 using 86011-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAP1GAP antibody (86011-1-RR, Clone: 250422B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Jurkat using 86011-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAP1GAP antibody (86011-1-RR, Clone: 250422B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A549-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86011-1-RR bindet in IF/ICC, ELISA RAP1GAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAP1GAP fusion protein Ag5576
• Vollständiger Name: RAP1 GTPase activating protein
• Berechnetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC054490
• Gene symbol: RAP1GAP
• Gene ID (NCBI): 5909
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rap1 GTPase-activating protein (Rap1GAP) is an important tumor suppressor. Rap1GAP was associated with the AMPK and NF-κB signaling pathways and that Rap1GAP inhibited AMPK/SIRT1 and activated the NF-κB signaling pathway in model animals. Rap1GAP was verified to regulate the AMPK SIRT1/NF-κB signaling pathway and exacerbate the damage to myocardial cells caused by ischemia and hypoxia (PMID: 38320624). Rap1GAP is involved in Ang II-induced cardiomyocyte hypertrophy through the regulation of autophagy (PMID: 33936386). Rap1GAP, which is the GTPase-activating protein of Rap1, can bind to Rap1, promote the transformation of GTP from its active to inactive form, and terminate signal transduction (PMID: 21295042).