RAPGEF2 Rekombinanter Antikörper

RAPGEF2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241637G5

Kat-Nr. : 84331-4-PBS

Synonyme

PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313, CNrasGEF

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  WB analysis using 84331-4-RR (same clone as 84331-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84331-4-RR (RAPGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84331-4-RR (same clone as 84331-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84331-4-RR (RAPGEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84331-4-RR (same clone as 84331-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAPGEF2 antibody (84331-4-RR, Clone: 241637G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84331-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84331-4-RR against Human RAPGEF2 were performed. The affinity constant is 0.19 nM.

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Geprüfte Anwendungen

Produktinformation

84331-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RAPGEF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAPGEF2 fusion protein Ag35288
• Vollständiger Name: Rap guanine nucleotide exchange factor (GEF) 2
• Berechnetes Molekulargewicht: 167kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_014247.2
• Gene symbol: RAPGEF2
• Gene ID (NCBI): 9693
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.