RAPGEF5 Polyklonaler Antikörper

RAPGEF5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12556-1-AP

Synonyme

GFR, KIAA0277, M Ras regulated Rap GEF, MR GEF, MRGEF, RAPGEF5, Related to Epac, REPAC

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  WB analysis of human brain using 12556-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12556-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12556-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12556-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12556-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12556-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• RIP: See 1 publications below

Produktinformation

12556-1-AP bindet in WB, RIP, ELISA RAPGEF5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAPGEF5 fusion protein Ag3229
• Vollständiger Name: Rap guanine nucleotide exchange factor (GEF) 5
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68-75 kDa
• GenBank-Zugangsnummer: BC039203
• Gene symbol: RAPGEF5
• Gene ID (NCBI): 9771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,RIP	Redox Biol; CircRAPGEF5 interacts with RBFOX2 to confer ferroptosis resistance by modulating alternative splicing of TFRC in endometrial cancer; Authors - Jun Zhang