RARA Polyklonaler Antikörper

RARA Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10331-1-AP

Synonyme

NR1B1, RAR, RAR alpha, RARA, Retinoic acid receptor alpha, retinoic acid receptor, alpha

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 10331-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10331-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10331-1-AP

  IP result of anti-RARA (IP:10331-1-AP, 4ug; Detection:10331-1-AP 1:300) with HeLa cells lysate 2600 ug.

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10331-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA RARA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RARA fusion protein Ag0420
• Vollständiger Name: retinoic acid receptor, alpha
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC008727
• Gene symbol: RARA
• Gene ID (NCBI): 5914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Br J Cancer; All-trans retinoic acid enhances the cytotoxic effect of decitabine on myelodysplastic syndromes and acute myeloid leukaemia by activating the RARα-Nrf2 complex; Authors - Lu Wang
mouse	WB,IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
human	WB	Front Cell Dev Biol; Silybin Prevents Prostate Cancer by Inhibited the ALDH1A1 Expression in the Retinol Metabolism Pathway.; Authors - Ying Jiang
human	WB	Cell Signal; ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury.; Authors - Zhen Yu
human	IF	Andrology; A novel SNP in HUWE1 promoter confers increased risk of NOA by affecting the RA/RARα pathway in Chinese individuals; Authors - Xudong Shan
human	WB,CoIP	Biomedicines; Histone H3 Acetylation Is Involved in Retinoid Acid-Induced Neural Differentiation through Increasing Mitochondrial Function; Authors - Yang Zhang