RARG Polyklonaler Antikörper

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• KD/KO Validated

RARG Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11424-1-AP

Synonyme

NR1B3, RAR gamma, RARC, RARG, Retinoic acid receptor gamma, retinoic acid receptor, gamma

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11424-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11424-1-AP (RARG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11424-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11424-1-AP (RARG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 11424-1-AP

  IP Result of anti-RARG (IP:11424-1-AP, 4ug; Detection:11424-1-AP 1:300) with MCF-7 cells lysate 1120ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

11424-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA RARG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RARG fusion protein Ag1991
• Vollständiger Name: retinoic acid receptor, gamma
• Berechnetes Molekulargewicht: 454 aa, 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC019098
• Gene symbol: RARG
• Gene ID (NCBI): 5916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoic acid receptors (RARs) are nuclear hormone receptors that act as ligand-dependent transcriptional regulators. In their liganded state, RARs activate transcription, whereas in their nonliganded form, they repress transcription of their target genes. RARs have numerous target genes, which have retinoic response elements in their promoter regions [PMID:17574023]. RARG is a member of the retinoid acid receptor (RAR) family, along with RARA, which is known to be involved PML/RARA fusion of acute promyelocytic leukemia, and RARB. and rearrangement that conferred to leukemic cells acute promyelocytic leukemia-like morphologic and immunophenotypic features [PMID:20935257].

Publikationen

Species	Application	Title
mouse	ChIP	J Clin Invest; The orphan nuclear receptor small heterodimer partner mediates male infertility induced by diethylstilbestrol in mice.; Authors - Volle David H DH
human	WB,IHC	Front Oncol; High expression of RARG accelerates ovarian cancer progression by regulating cell proliferation; Authors - Lin Xiu ; KD Validated
human	IF	Endocrinology; De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.; Authors - Tomoko Kawai
mouse	ChIP	Adv Sci (Weinh); Synthetic Retinoid Kills Drug-Resistant Cancer Stem Cells via Inducing RARγ-Translocation-Mediated Tension Reduction and Chromatin Decondensation; Authors - Yao Zhang