RARG Rekombinanter Antikörper

RARG Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250399C1

Kat-Nr. : 86098-1-RR

Synonyme

NR1B3, RAR gamma, RARC, RAR-gamma, Retinoic acid receptor gamma

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  WB analysis using 86098-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86098-1-RR (RARG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 86098-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RARG antibody (86098-1-RR, Clone: 250399C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A431-Zellen, HeLa-Zellen, NCCIT-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86098-1-RR bindet in WB, ELISA RARG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RARG fusion protein Ag1991
• Vollständiger Name: retinoic acid receptor, gamma
• Berechnetes Molekulargewicht: 454 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50-60kDa
• GenBank-Zugangsnummer: BC019098
• Gene symbol: RARG
• Gene ID (NCBI): 5916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoic acid receptors (RARs) are nuclear hormone receptors that act as ligand-dependent transcriptional regulators. In their liganded state, RARs activate transcription, whereas in their nonliganded form, they repress transcription of their target genes. RARs have numerous target genes, which have retinoic response elements in their promoter regions (PMID:17574023). RARG is a member of the retinoid acid receptor (RAR) family, along with RARA, which is known to be involved PML/RARA fusion of acute promyelocytic leukemia, and RARB. and rearrangement that conferred to leukemic cells acute promyelocytic leukemia-like morphologic and immunophenotypic features (PMID:20935257).