Chemerin Polyklonaler Antikörper

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• KD/KO Validated

Chemerin Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 10216-1-AP

Synonyme

Chemerin, HP10433, RAR responsive protein TIG2, RARRES2, TIG2

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 10216-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 10216-1-AP (Chemerin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Produktinformation

10216-1-AP bindet in WB, IHC, ELISA Chemerin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Chemerin fusion protein Ag0261
• Vollständiger Name: retinoic acid receptor responder (tazarotene induced) 2
• Berechnetes Molekulargewicht: 163 aa, 19 kDa
• Beobachtetes Molekulargewicht: 14-19 kDa
• GenBank-Zugangsnummer: BC000069
• Gene symbol: RARRES2
• Gene ID (NCBI): 5919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. RARRES2 have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. The MW of Chemerin is 14-19 kDa.

Publikationen

Species	Application	Title
mouse,human	IHC,WB	Mil Med Res; RARRES2 regulates lipid metabolic reprogramming to mediate the development of brain metastasis in triple negative breast cancer; Authors - Yi-Qun Li
human	WB	Cancer Cell Int; Targeting copper death genotyping associated gene RARRES2 suppresses glioblastoma progression and macrophages infiltration; Authors - Tao Yan
human,mouse	WB,IHC	Front Pharmacol; Targeting the chemerin/CMKLR1 axis by small molecule antagonist α-NETA mitigates endometriosis progression; Authors - Ming Yu ; KO Validated
human	IHC	Front Oncol; Neutrophils Promote Tumor Progression in Oral Squamous Cell Carcinoma by Regulating EMT and JAK2/STAT3 Signaling Through Chemerin.; Authors - Xiaoyuan Hu
human	WB	J Zhejiang Univ Sci B; Silencing of DsbA-L gene impairs the PPARγ agonist function of improving insulin resistance in a high-glucose cell model.; Authors - Xuan Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Owen (Verified Customer) (10-07-2019)

The antibody manages to detect the correct band of the protein and is specific. Further optimization for IHC would be great.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: 786-O cell