RARS Polyklonaler Antikörper

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• KD/KO Validated

RARS Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 27344-1-AP

Synonyme

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

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  WB analysis of HeLa using 27344-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27344-1-AP

  WB result of RARS antibody (27344-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells.

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  WB analysis of A431 using 27344-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 27344-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 27344-1-AP

  IP result of anti-RARS (IP:27344-1-AP, 4ug; Detection:27344-1-AP 1:500) with HeLa cells lysate 3200 ug.

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  IHC staining of human heart using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, U-251-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below

Produktinformation

27344-1-AP bindet in WB, IHC, IP, ELISA RARS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RARS fusion protein Ag26390
• Vollständiger Name: arginyl-tRNA synthetase
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC000528
• Gene symbol: RARS
• Gene ID (NCBI): 5917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants; Authors - Chao Yang ; KD Validated
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
human	WB	bioRxiv; Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells; Authors - Dennis J Hsu ; KD Validated
human	WB	Sci Adv; Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells; Authors - Dennis J Hsu ; KD Validated
rat	WB	Mol Neurobiol; Verification of Pain-Related Neuromodulation Mechanisms of Calcitonin in Knee Osteoarthritis; Authors - Qing Lin