RASGAP Polyklonaler Antikörper

RASGAP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 12935-1-AP

Synonyme

RASA1, Ras p21 protein activator, Ras GTPase-activating protein 1, p120RASGAP, p120GAP

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  WB analysis using 12935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12935-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12935-1-AP

  IP result of anti-RASGAP (IP:12935-1-AP, 4ug; Detection:12935-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

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  IHC staining of human testis using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12935-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RASGAP antibody (12935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

12935-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RASGAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RASGAP fusion protein Ag3906
• Vollständiger Name: RAS p21 protein activator (GTPase activating protein) 1
• Berechnetes Molekulargewicht: 1047 aa, 116 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 95 kDa
• GenBank-Zugangsnummer: BC033015
• Gene symbol: RASA1
• Gene ID (NCBI): 5921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Aging (Albany NY); LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.; Authors - Liting Jin
	WB	Am J Transl Res; GRK2 deletion improves the function of skin flap following ischemia-reperfusion injury by regulating Drp1.; Authors - Yapeng Wang
rat	WB	Biochem Biophys Res Commun; miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.; Authors - Wang Junfeng J
human	WB	Int J Clin Exp Pathol; Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.; Authors - Xiaojuan Ye
		Funct Integr Genomics; Amlodipine inhibits Synaptotagmin-4's oncogenic activity on gastric cancer proliferation by targeting calcium signaling; Authors - Wen Huang
human	WB	Cancer Res; Blocking Nitrosylation Induces Immunogenic Cell Death by Sensitizing NRAS-Mutant Melanoma to MEK Inhibitors; Authors - Jyoti Srivastava