RASA2 Polyklonaler Antikörper

RASA2 Polyklonal Antikörper für IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31586-1-AP

Synonyme

GAP1M, RAS p21 protein activator 2, RASGAP

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  IP experiment of Jurkat using 31586-1-AP

  IP result of anti-RASA2 (IP:31586-1-AP, 4ug; Detection:31586-1-AP 1:1000) with Jurkat cells lysate 1920 ug.

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  IP experiment of A431 using 31586-1-AP

  IP result of anti-RASA2 (IP:31586-1-AP, 4ug; Detection:31586-1-AP 1:1000) with A431 cells lysate 1120 ug.

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  IF Staining of A431 using 31586-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASA2 antibody (31586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche IP: Jurkat-Zellen, A431-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31586-1-AP bindet in IF/ICC, IP, ELISA RASA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RASA2 fusion protein Ag35885
• Vollständiger Name: RAS p21 protein activator 2
• Berechnetes Molekulargewicht: 96kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: NM_001303245
• Gene symbol: RASA2
• Gene ID (NCBI): 5922
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ras GTPase-activating protein 2 (RASA2) is also named GAP1M and RASGAP. RASA2 was a critical RAS-specific GAP, inhibition of which permitted increased proliferation following TCR stimulation under conditions of immunosuppression (PMID: 37858490). RASA2 was identified as an inhibitor of RAS signaling in multiple human tumors, including melanoma, where inactivating RASA2 mutations have been found (PMID: 26502337). RASA2 is also one of the genes mutated in Noonan syndrome, a Rasopathy associated with developmental defects and elevated RAS activity (PMID: 25049390). RASA2 is expressed in both CD4+ and CD8+ T cells (PMID: 36002574).