RASGRF1 Monoklonaler Antikörper

RASGRF1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A4D4

Kat-Nr. : 67010-1-PBS

Synonyme

1A4D4, CDC25, CDC25L, GNRP, GRF1

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  WB analysis of pig cerebellum using 67010-1-Ig (same clone as 67010-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67010-1-Ig (same clone as 67010-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67010-1-Ig (same clone as 67010-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67010-1-Ig (same clone as 67010-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67010-1-PBS bindet in WB, Indirect ELISA RASGRF1 und zeigt Reaktivität mit Hausschwein, Ratten

• Getestete Reaktivität: Hausschwein, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RASGRF1 fusion protein Ag6937
• Vollständiger Name: Ras protein-specific guanine nucleotide-releasing factor 1
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC040275
• Gene symbol: RASGRF1
• Gene ID (NCBI): 5923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.