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Phospho-RB1 (Ser780) Rekombinanter Antikörper

Phospho-RB1 (Ser780) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241827H7

Kat-Nr. : 84692-1-PBS

Synonyme

pRb, RB1, pp110, p110-RB1 , p110-RB1

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-RB1 (Ser780) Recombinant antibody (84692-1-RR)

    WB analysis using 84692-1-RR (same clone as 84692-1-PBS)

    Non-treated K-562 cells, phosphatase inhibitor treated K-562 cells and λ phosphatase treated K-562 cells were subjected to SDS PAGE followed by western blot with 84692-1-RR (Phospho-RB1 (Ser780) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84692-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84692-1-PBS bindet in WB, Indirect ELISA Phospho-RB1 (Ser780) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name retinoblastoma 1
Berechnetes Molekulargewicht 928 aa, 106 kDa
Beobachtetes Molekulargewicht110 kDa
GenBank-ZugangsnummerBC039060
Gene symbol RB1
Gene ID (NCBI) 5925
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1.