RB1CC1 Rekombinanter Antikörper

RB1CC1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242204D6

Kat-Nr. : 84757-3-PBS

Synonyme

242204D6, CC1, FAK family kinase-interacting protein of 200 kDa, KIAA0203, RB1CC 1

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  WB analysis using 84757-3-RR (same clone as 84757-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84757-3-RR (RB1CC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84757-3-RR (same clone as 84757-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84757-3-RR (RB1CC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84757-3-RR (same clone as 84757-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84757-3-RR (RB1CC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84757-3-RR (same clone as 84757-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RB1CC1 antibody (84757-3-RR, Clone: 242204D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84757-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84757-3-RR against Human RB1CC1 were performed. The affinity constant is 3.20 nM.

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Geprüfte Anwendungen

Produktinformation

84757-3-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RB1CC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RB1CC1 fusion protein Ag10508
• Vollständiger Name: RB1-inducible coiled-coil 1
• Berechnetes Molekulargewicht: 1594 aa, 183 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC017556
• Gene symbol: RB1CC1
• Gene ID (NCBI): 9821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene.