RBBP6 Polyklonaler Antikörper

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• KD/KO Validated

RBBP6 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11882-1-PBS

Synonyme

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

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  WB analysis using 11882-1-AP (same clone as 11882-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11882-1-AP (same clone as 11882-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 11882-1-AP (same clone as 11882-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 11882-1-AP (same clone as 11882-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11882-1-AP (same clone as 11882-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11882-1-PBS bindet in WB, IHC, Indirect ELISA RBBP6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBBP6 fusion protein Ag2484
• Vollständiger Name: retinoblastoma binding protein 6
• Berechnetes Molekulargewicht: 1792 aa, 200 kDa
• Beobachtetes Molekulargewicht: 250 kDa
• GenBank-Zugangsnummer: BC029352
• Gene symbol: RBBP6
• Gene ID (NCBI): 5930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].