RbAp46 Rekombinanter Antikörper

RbAp46 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241189D6

Kat-Nr. : 84330-1-PBS

Synonyme

RBBP7, 241189D6, Histone-binding protein RBBP7, Nucleosome-remodeling factor subunit RBAP46, RBBP-7

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  WB analysis using 84330-1-RR (same clone as 84330-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84330-1-RR (RBBP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84330-1-RR (same clone as 84330-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBBP7 antibody (84330-1-RR, Clone: 241189D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84330-1-RR (same clone as 84330-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RBBP7 Recombinant antibody (84330-1-RR, Clone:241189D6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84330-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA RbAp46 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: retinoblastoma binding protein 7
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_002893
• Gene symbol: RBBP7
• Gene ID (NCBI): 5931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.