RBBP9 Polyklonaler Antikörper

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• KD/KO Validated

RBBP9 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12230-2-AP

Synonyme

B5T-overexpressed gene protein, BOG, EC:3.-.-.-, Glutathione S-Transferase, RBBP 10

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  WB analysis of mouse testis using 12230-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12230-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12230-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12230-2-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12230-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12230-2-AP

  IP result of anti-RBBP9 (IP:12230-2-AP, 3ug; Detection:12230-2-AP 1:300) with mouse lung tissue lysate 5160ug.

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  IHC staining of human pancreas cancer using 12230-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12230-2-AP (RBBP9 antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of BxPC-3 using 12230-2-AP

  1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug RBBP9 Polyclonal antibody (12230-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, A375-Zellen, humanes Lungengewebe, humanes Milzgewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

12230-2-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA RBBP9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBBP9 fusion protein Ag2868
• Vollständiger Name: retinoblastoma binding protein 9
• Berechnetes Molekulargewicht: 186 aa, 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC015938
• Gene symbol: RBBP9
• Gene ID (NCBI): 10741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID: 20080647) The genes CyFIP2 and RbBP9, which are also miss-expressed in ALS hMSC, could serve as diagnostic biomarker tools for detection of ALS in blood samples.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Proc Natl Acad Sci U S A; RBBP9: a tumor-associated serine hydrolase activity required for pancreatic neoplasia.; Authors - Shields David J DJ
mouse	WB,IHC	Cell Mol Gastroenterol Hepatol; Retinoblastoma-Binding Protein 9 Suppresses Intestinal Inflammation and Inflammation-Induced Tumorigenesis in Mice; Authors - Kensuke Hamada ; KD Validated
mouse,human	WB	Antioxid Redox Signal; Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.; Authors - Maya A Olshina
human	WB	Exp Hematol; Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance.; Authors - Michael D O'Connor ; KD Validated
human	WB	Dis Markers; Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis.; Authors - Nachmany Henny H