RBBP9 Monoklonaler Antikörper

RBBP9 Monoklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H6D8

Kat-Nr. : 66015-1-Ig

Synonyme

RBBP9

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  WB analysis of A549 using 66015-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66015-1-Ig (RBBP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66015-1-Ig(RBBP9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66015-1-Ig(RBBP9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 66015-1-Ig

  Immunofluorescent analysis of Hela cells, using RBBP9 antibody 66015-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66015-1-Ig bindet in WB, IF, IHC, ELISA RBBP9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBBP9 fusion protein Ag17864
• Vollständiger Name: retinoblastoma binding protein 9
• Berechnetes Molekulargewicht: 186 aa, 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC015938
• Gene symbol: RBBP9
• Gene ID (NCBI): 10741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell–cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)