RBM14 Polyklonaler Antikörper

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• KD/KO Validated

RBM14 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 10196-1-AP

Synonyme

RNA-binding protein 14, RNA-binding motif protein 14, RNA binding protein 14, PSP2, Paraspeckle protein 2

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  WB analysis of Jurkat using 10196-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10196-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

10196-1-AP bindet in WB, IF, ELISA RBM14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM14 fusion protein Ag0254
• Vollständiger Name: RNA binding motif protein 14
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC000488
• Gene symbol: RBM14
• Gene ID (NCBI): 10432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM14 is a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation.

Publikationen

Species	Application	Title
	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Exp Mol Med; The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability; Authors - Qianqian Yang
human	WB	Brain Res; Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network.; Authors - Yang Li
human	WB	Transl Cancer Res; Molecular mechanism of RBM14-mediated promotion of proliferation, migration, and invasion in osteosarcoma; Authors - Sijian Lin ; KD Validated
pig,human	WB,IF	Int J Biol Macromol; Subcellular proteomics reveals the crosstalk between nucleocytoplasmic trafficking and the innate immune response to Senecavirus A infection; Authors - Mingyang Li
pig	WB	J Virol; RBM14 inhibits the replication of porcine epidemic diarrhea virus by recruiting p62 to degrade nucleocapsid protein through the activation of autophagy and interferon pathway; Authors - Xiaoquan Wang

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