RBM16 Polyklonaler Antikörper

RBM16 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18893-1-AP

Synonyme

CCAP7, KIAA1116, RBM16, RNA binding motif protein 16, RNA binding protein 16, SCAF8

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Galerie der Validierungsdaten

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  WB analysis of HT-1080 using 18893-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18893-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18893-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 18893-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 18893-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18893-1-AP

  IP Result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug.

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  IHC staining of human testis using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 18893-1-AP

  Immunofluorescent analysis of Hela cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 18893-1-AP

  Immunofluorescent analysis of HepG2 cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HT-1080.Zellen, L02-Zellen, NIH/3T3-Zellen, THP-1-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18893-1-AP bindet in WB, IP, IHC, IF, ELISA RBM16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM16 fusion protein Ag13499
• Vollständiger Name: RNA binding motif protein 16
• Berechnetes Molekulargewicht: 1271 aa, 141 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC070071
• Gene symbol: RBM16
• Gene ID (NCBI): 22828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )