RBM16 Polyklonaler Antikörper

RBM16 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18893-1-PBS

Synonyme

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  WB analysis of HT-1080 using 18893-1-AP (same clone as 18893-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis using 18893-1-AP (same clone as 18893-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 18893-1-AP (same clone as 18893-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 18893-1-AP (same clone as 18893-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 18893-1-AP (same clone as 18893-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

  IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM16 antibody (18893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18893-1-AP (same clone as 18893-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18893-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RBM16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM16 fusion protein Ag13499
• Vollständiger Name: RNA binding motif protein 16
• Berechnetes Molekulargewicht: 1271 aa, 141 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC070071
• Gene symbol: RBM16
• Gene ID (NCBI): 22828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )