RBM17 Rekombinanter Antikörper

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• KD/KO Validated

RBM17 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230125A10

Kat-Nr. : 82889-2-PBS

Synonyme

230125A10, 45 kDa-splicing factor, RBM 17, RNA-binding motif protein 17, SPF 45

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  WB analysis of MCF-7 using 82889-2-RR (same clone as 82889-2-PBS)

  WB result of RBM17 antibody (82889-2-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells. This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 82889-2-RR (same clone as 82889-2-PBS)

  IP result of anti-RBM17 (IP:82889-2-RR, 4ug; Detection:82889-2-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82889-2-RR (same clone as 82889-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM17 antibody (82889-2-RR, Clone: 230125A10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 82889-2-RR (same clone as 82889-2-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human RBM17 (82889-2-RR, Clone:230125A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82889-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82889-2-RR against Human RBM17 were performed. The affinity constant is 2.86 nM.

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Geprüfte Anwendungen

Produktinformation

82889-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA RBM17 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBM17 fusion protein Ag0985
• Vollständiger Name: RNA binding motif protein 17
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC007871
• Gene symbol: RBM17
• Gene ID (NCBI): 84991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.