RBM20 Rekombinanter Antikörper

RBM20 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240345B7

Kat-Nr. : 83363-1-RR

Synonyme

RNA binding protein, RNA binding motif protein 20, 240345B7

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  WB analysis using 83363-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83363-1-RR (RBM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 83363-1-RR

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug RBM20 Recombinant antibody (83363-1-RR, Clone:240345B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83363-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83363-1-RR against Human RBM20 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, A549-Zellen, K-562-Zellen
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83363-1-RR bindet in WB, FC (Intra), ELISA RBM20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBM20 fusion protein Ag34322
• Vollständiger Name: RNA binding motif protein 20
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: 190 kDa
• GenBank-Zugangsnummer: NM_001134363
• Gene symbol: RBM20
• Gene ID (NCBI): 282996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543).